Want to know more about macromolecular crystallography?

October 30, 2025

We've published a paper in Nature Reviews Primer together with Pavel V. Afonine, Armando Albert, Kay Diederichs, Juan A. Hermoso, Eugene Krissinel, José Antonio Márquez, Santosh Panjikar, Maria Solà & Isabel Usón.

Nature Reviews Methods Primers: Macromolecular crystallography

This Primer offers a practical and rational introduction to macromolecular crystallography. Andrea has written the part about critically assessing structures and data.

a, A 2Fo – Fc electron density map contoured at 1σ for the open (apo state) of AliD protein of Streptococcus pneumoniae (PDB ID 8QLC) solved at 1.80 Å resolution. b, A 2Fo – Fc electron density map contoured at 1σ for AliD in complex with FPPQSV (PDB ID 8QLG) at 1.98 Å resolution. c, A zoomed-in view showing the substrate-binding site of AliD (cyan sticks) in complex with the oligopeptide substrate (green sticks) (PDB ID 8QLG). d, A 2Fo – Fc electron density map contoured at 1σ (blue mesh) and a Fo – Fc map contoured at 2.5σ (green mesh) for the AliB protein of S. pneumoniae (salmon sticks) in complex with unknown oligopeptides (PDB ID 8QLJ) at 1.65 Å resolution. An explanation of the map coefficients (such as 2Fo – Fc) is provided in Box 1.

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